ISK-015

TNFRSF13C; TNFSF13B

ID Components Size (96 tests)
ISK-015-1 High-bind Plate 1 plate
ISK-015-2 Human BAFF 20 μg
ISK-015-3 Biotinylated Human BAFFR 10 μg
ISK-015-4 Anti-BAFFR Neutralizing Antibody 20 μg
ISK-015-5 Streptavidin-HRP 10 μg
ISK-015-6 Coating Buffer 12 mL
ISK-015-7 10xWashing Buffer 50 mL
ISK-015-8 Blocking Buffer 50 mL
ISK-015-9 Substrate Solution 24 mL
ISK-015-10 Stop Solution 7 mL

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

96 tests

10-0.02μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new BAFFR pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human BAFFR to immobilized human BAFF in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinlated BAFFR protein, a human BAFF protein, an anti-BAFFR neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human BAFF.
b) Add your molecule of interest to the tests.
c) Add human BAFFR-Biotin to bind the coated human BAFF.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to BAFFR: BAFF binding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of human BAFFR binding to human BAFF

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Serial dilutions of Anti-BAFFR Neutralizing antibody (1:1 serial dilution, from 10 μg/mL to 0.02μg/mL) was added into BAFF: Biotinlated BAFFR binding reactions. The assay was performed according to the protocol described above. Background was subtracted from data points prior to log transformation and curve fitting.