HEK293/CHO Stable Cell Line Development

Creative BioMart offers stable cell line development service, including HEK293 and CHO stable cell lines for immune checkpoint research assay as well as industrial production. We increase stable cell line productivity effectively and provide one-stop service from gene sequence to deliverable stable cell line.

Why develop HEK293/CHO stable cell lines

HEK293/CHO stable cell lines are widely applied in functional studies of immune checkpoint genes, drug screening and production of proteins.

Functional studies: the expression level of an immune checkpoint gene in certain cells can be manipulated to learn its functions.
Immune checkpoint inhibitors screening: Stable cell lines are quite essential for immune checkpoint inhibitors screening in a combinatorial chemical library.
Immune checkpoint proteins for research: Immune checkpoint proteins for research purposes can be produced through transient transfection of HEK293/CHO cells. However, this method is not very effective in producing a large amount of recombinant immune checkpoint proteins. Therefore, a stable HEK293/CHO cell line is more desirable.
Immune checkpoint proteins/ antibodies for pharmaceutical: Stable cell lines are very important for FDA approval and suitable for preparing immune checkpoint proteins, antibodies for therapeutic purposes.

How to develop stable cells

HEK293 stable cell line development

Service process	Description	Time	Deliverables
Gene synthesis and codon optimization		1-2 weeks
Vector construction	1. Cloning cDNA into vector 2. Plasmid sequencing 3. Large-scale plasmid preparation	2-3 weeks	Sequence report
Transient expression evaluation	1. Transient expression 2. Level detection by ELISA/WB	2 weeks	Evaluation report
Stable cell line development	1. Stable transfection and screening 2. High expression clone screening and stability screening 3. Cell bank construction	12-20 weeks	Report and stable clones
Large-scale expression and purification		2-3 weeks	Purified protein, SDS-PAGE report and Co.A

CHO stable cell line development

Service process	Description	Time	Deliverables
Gene synthesis and codon optimization		1-2 weeks
Vector construction	1. Cloning cDNA into vector 2. Plasmid sequencing 3. Large-scale plasmid preparation	2-3 weeks	Sequence report
Transient expression evaluation	1. Transient expression 2. Level detection by ELISA/WB	2 weeks	Evaluation report
Stable cell line development	1. Stable transfection and screening 2. High expression clone screening and stability screening 3. Cell bank construction	10-12 weeks	Report and 2 tube cells
Large-scale expression and purification		2-3 weeks	Purified protein, SDS-PAGE report and Co.A

HEK293/CHO stable cell development processes

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