Immune Checkpoint Inhibitors Binding and Interaction Assay

Usually, the immune checkpoint inhibitors perform their functions through blocking interactions between immune checkpoint proteins and corresponding ligands. Creative BioMart provides customers with immune checkpoint inhibitors binding and interaction assay service to test specificity of inhibitors to their targets. We will use ELISA and Creative BioMart cell line-based binding assay in this service.

ELISA

Enzyme-Linked Immunosorbent Assay (ELISA) is a very commonly used immunoassay, which can detect and quantify antibodies, peptides, proteins and small molecules by using a multi-well plate. Direct-ELISA is the most used to estimate antigen cross-reactivity. Creative BioMart will discuss with reseach teams of customers and make an experimental plan to evaluate your immune checkpoint (PD-1, CTLA-4, etc.) inhibitors binding to the ligands or receptors through ELISA assay.

Figure 1. Types of ELISA.

Cell line based assay

In this method, we firstly use flow cytometry tool to measure the binding and interaction of immune checkpoint inhibitors with cell lines which can express targeted immune checkpoint proteins. Then, EC50 (binding affinities) and IC50(binding inhibitiong) can be generated with immune checkpoint inhibitors provided from customers.

Strenghs of our service

• We have experienced immunologists to perform strict assays and high-quality reagents as well as equipments.
• We have certain cell line library which can express various immune checkpoint proteins for immune checkpoint inhibitors binding and interaction assays.
• Combined with our immune checkpoint funcational assay, we are devoted to help customers to desgin your own immnue checkpoint inhibitor roadmap.
• Combined with our immune checkpoint antibody discovery service, we are your ideal CRO for immune checkpoint inhibitors development for cancer immunothrapy candidates.