GMP Recombinant Human CD27 protein(NP_001233.1)(394 aa), fused with Fc Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.
Expression System
HEK293
Species
Human
Tag
C-hFc Tag
Form
Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
Molecular Mass
Recombinant Human CD27 protein contains 394 aa. It has a predicted MW of 46.2 kDa.
Protein length
DNA sequence encoding Human CD27(NCBI Reference Sequence: NP_001233.1) was expressed with Fc tag at the C-terminal.
Endotoxin
<0.1EU/ug
Purity
>=95% by SDS-PAGE and HPLC
Storage
The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.