ISK-021

IL17A; IL17RA

ID Components Size (96 tests)
ISK-021-1 High-bind Plate 1 plate
ISK-021-2 Human IL-17A 10 μg
ISK-021-3 Biotinylated Human IL-17RA 10 μg
ISK-021-4 Anti-IL-17A Neutralizing Antibody 20 μg
ISK-021-5 Streptavidin-HRP 10 μg
ISK-021-6 Coating Buffer 12 mL
ISK-021-7 10xWashing Buffer 50 mL
ISK-021-8 Blocking Buffer 50 mL
ISK-021-9 Substrate Solution 12 mL
ISK-021-10 Stop Solution 12 mL

Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

96 tests

20-0.01μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-17A pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-17RA to immobilized human IL-17A in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-17RA protein, a human IL-17A protein, an anti-IL-17A neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-17A.
b) Add your molecule of interest to the tests.
c) Add human IL-17 RA-Biotin to bind the coated IL-17 A.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-17 RA: IL-17A binding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of human IL-17A binding to human IL-17RA.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.