ISK-020

IL17A; IL17RA

ID Components Size (96 tests)
ISK-020-1 High-bind Plate 1plate
ISK-020-2 Human IL-17RA 15μg
ISK-020-3 Biotinylated Human IL-17A 10μg
ISK-020-4 Anti-IL-17A Neutralizing Antibody 20μg
ISK-020-5 Streptavidin-HRP 10μg
ISK-020-6 Coating Buffer 12mL
ISK-020-C7 10xWashing Buffer 50mL
ISK-020-8 Blocking Buffer 50mL
ISK-020-9 Substrate Solution 12mL
ISK-020-10 Stop Solution 7mL

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8), which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also, IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). Furthermore, IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

96 tests

10-0.01μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-17A pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-17A to immobilized human IL-17 RA in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-17A protein, a human IL-17 RA protein, an anti-IL-17A neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-17 RA.
b) Add your molecule of interest to the tests.
c) Add human IL-17A-Biotin to bind the coated IL-17 RA.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-17A: IL-17 RA binding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of human IL-17A binding to human IL-17RA.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.