ISK-026

IL4R; IL4

ID Components Size (96 tests)
ISK-026-1 High-bind Plate 1 plate
ISK-026-2 Human IL-4 25 μg
ISK-026-3 Biotinylated Human IL-4 Rα 10 μg
ISK-026-4 Anti-IL-4Rα Neutralizing Antibody 20 μg
ISK-026-5 Streptavidin-HRP 10 μg
ISK-026-6 Coating Buffer 12 mL
ISK-026-7 10xWashing Buffer 50 mL
ISK-026-8 Blocking Buffer 50 mL
ISK-026-9 Substrate Solution 12 mL
ISK-026-10 Stop Solution 7 mL

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.

96 tests

5-0.00244μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-4 Rα pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-4 Rα to immobilized human IL-4 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-4 Rα protein, a human IL-4 protein, an anti-IL-4Rα neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-4.
b) Add your molecule of interest to the tests.
c) Add human IL-4 Rα-Biotin to bind the coated IL-4.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-4 Rα: IL-4 binding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of human IL-4Rα binding to human IL-4.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Serial dilutions of Anti-IL-4 Rα Neutralizing antibody (1:1 serial dilution, from 5 μg/mL to 0.00244μg/mL) was added into Biotinylated IL-4Rα:IL-4 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting.