ISK-017

IL6; IL6R

ID Components Size (96 tests)
ISK-017-1 High-bind Plate 1 plate
ISK-017-2 Human IL-6 R alpha 15 μg
ISK-017-3 Biotinylated Human IL-6 10 μg
ISK-017-4 Anti-IL-6 Neutralizing Antibody 10 μg
ISK-017-5 Streptavidin-HRP 10 μg
ISK-017-6 Coating Buffer 12 mL
ISK-017-7 10xWashing Buffer 50 mL
ISK-017-8 Blocking Buffer 50 mL
ISK-017-9 Substrate Solution 12 mL
ISK-017-10 Stop Solution 7 mL

Interleukin 6 (IL-6) is also known as HGF, BSF2, HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages, monocytes, fibroblasts, endothelial cells, et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes, atherosclerosis, depression, Alzheimers Disease, systemic, lupus erythematosus, prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

96 tests

10-0.01μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-6 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-6 to immobilized human IL-6 R alpha in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-6 protein, a human IL-6 R alpha protein, an anti-IL-6 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-6 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-6-Biotin to bind the coated IL-6 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-6: IL-6 R alpha binding will be determined by comparing OD readings among different experimental groups.

This kit is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product by various biotechnological processes such as cell or tissue culture.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Serial dilutions of Anti-IL-6 Neutralizing antibody (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting.