Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Activity
Immobilized Mouse IL-13Ra2, His tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for human IL-13, hFc tag with the EC50 of 11.5 ng/mL determined by ELISA (QC Test).
Product Background
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha, is now referred to as IL-13 R alpha 2.
