Description
PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body' immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.
Assay Principles
This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-1 to immobilized human PD-L1 in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform. Briefly, we provide you with a human PD-1-Biotin protein, a human PD-L1 protein, an anti-PD-1 neutralizing antibody (as method verified Std.), and streptavidin-HRP reagent. Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add Human PD-1-Biotin to bind the coated human PD-L1.
c) Add your molecule of interest to the tests.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.
Storage
Be sure to store each component at the proper temperature upon arrival. It is recommended not to freeze thaw more than 3 times. This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20ºC for 1 year in lyophilized state; -70ºC for 6 months under sterile conditions after reconstitution.