ISK-013

CD274; PDCD1

ID Components Size (96 tests)
ISK-013-1 High-bind Plate 1 plate
ISK-013-2 Human PD-L1 10 μg
ISK-013-3 Biotinylated Human PD-1 30 μg
ISK-013-4 Anti-PD-L1 Neutralizing Antibody 20 μg
ISK-013-5 Streptavidin-HRP 10 μg
ISK-013-6 Coating Buffer 12mL
ISK-013-7 10xWashing Buffer 50mL
ISK-013-8 Blocking Buffer 50mL
ISK-013-9 Substrate Solution 12mL
ISK-013-10 Stop Solution 7mL

Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.

96 tests

2-0.015625μg/mL

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-L1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-1 to immobilized human PD-L1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated PD-1 protein, a human PD-L1 protein, an anti-PD-L1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-L1: PD-1 binding will be determined by comparing OD readings among different experimental groups.

This kit is developed for screening for inhibitors of human PD-L1 binding to human PD-1.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Serial dilutions of Anti-PD-L1 Neutralizing antibody (1:1 serial dilution, from 2 μg/mL to 0.015625μg/mL) was added into PD-L1: Biotinylated PD-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting.