Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Activity
Immobilized human IL-13, His tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc tag with the EC50 of 22.6 ng/mL determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay.
Product Background
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha, is now referred to as IL-13 R alpha 2.
