Recombinant Canine CD47 protein, C-His Tag
Product Description
Cat
IMP-106
Official Symbol
CD47
Product Overview
Recombinant Canine CD47 protein(AA Gln 19-Asn 140)(Accession # F1P6D7-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.
Expression System
HEK293
Species
Canine
Tag
C-His Tag
Form
Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Molecular Mass
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Gln 19-Asn 140
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>90% as determined by reduced SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Canine CD47, His Tag with a linear range of 5-78 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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