Product Overview
Recombinant Canine HGF protein (Arg28-Arg494, Lys266Gln, Trp321Arg, Leu458Pro, alpha chain & Val495-Ser730, Ile693Thr, beta chain) without tag was expressed in Sf 21 cells.
Description
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss.
Protein length
Arg28-Arg494, Lys266Gln, Trp321Arg, Leu458Pro, alpha chain & Val495-Ser730, Ile693Thr, beta chain
Bio-activity
Measured in a cell proliferation assay using GGE canine gallbladder epithelial cells. The ED50 for this effect is 10-40 ng/mL. Measured by its ability to compete with biotinylated Recombinant Canine HGF for binding to immobilized Recombinant Canine HGF R/c-MET in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
