Recombinant Canine TIGIT protein, C-His Tag

Product Description

Cat

IMP-101

Official Symbol

TIGIT

Product Overview

Recombinant Canine TIGIT protein(AA Phe 17-Pro 208)(Accession # F1PKJ0-1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Canine

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 17-Pro 208

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Canine TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Canine TIGIT, His Tag at 5 ug/mL (100 uL/well) can bind Human CD155, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.01-0.313 ug/mL.

Bioactivity-BLI 1

Loaded Human CD155, Fc Tag on Protein A Biosensor, can bind Canine TIGIT, His Tag with an affinity constant of 5 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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