Recombinant Canine TNF Protein, No Tag
Product Description
Cat
IMP-4992
Official Symbol
TNF
Product Overview
Recombinant soluble form of canine TNFα (NP_001003244.4) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Description
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Expression System
E. coli
Species
Canine
Tag
No Tag
Predicted N Terminal
Met
Form
Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant canine TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.
Protein length
Val77-Leu233
Endotoxin
Please contact us for more information.
Purity
> 96 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1.2 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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