Recombinant Canine TNFRSF1A Protein (Ile22-Thr211), Tag Free
Product Description
Cat
IMP-9796
Official Symbol
TNFRSF1A
Product Overview
Recombinant Canine TNF RI/TNFRSF1A protein (Ile22-Thr211) with an N-terminal Met was expressed in E. coli.
Description
This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
Expression System
E. coli
Species
Canine
Tag
Tag Free
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 21.6 kDa
Protein length
Ile22-Thr211
Bio-activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 15 ng/mL of rcaTNF-alpha.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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