Recombinant Cat IL2 (C146S) Protein (21-154aa), N-His-Tagged

Product Description

Cat

IMP-9441

Official Symbol

IL2

Product Overview

Recombinant feline IL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Description

IL2, also known as interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytes, macrophages and oligodendrocytes.

Expression System

E. coli

Species

Cat

Tag

N-His

Form

Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Molecular Mass

17.8 kDa (157 aa) confirmed by MALDI-TOF

Protein length

21-154

AA Sequence

<MGSSHHHHHHSSGLVPRGSHMGS>APASSSTKETQQQLEQLLLDLRLLLNGVNNPENPKLSRMLTFKFYVPKKATELTHLQCLVEELKPLEEVLYLAQSKNFHLNHIKELMSNINVTVLKLKGSETRFTCNYDDETATIVEFLNKWITFCQSIFSTLT

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.25 mg/mL (determined by BCA assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Feline IL-2 (C146S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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