Cat IL2 (NP_001036802, 21 a.a.-154 a.a.) partial recombinant protein with His tag was expressed in Escherichia coli.
Expression System
E. coli
Species
Cat
Tag
His
Form
Liquid, In 20mM Tris-HCl, 0.15 M NaCl, pH 8.0 (0.1 mM DTT, 30% glycerol)
Molecular Mass
17.8 kDa
Protein length
21-154
Endotoxin
> 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE
Storage
Stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
SDS-PAGE
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Bioactivity-ELISA 1
Il-2 (Cat) Recombinant Protein stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤ 0.3 ng/mL.