Recombinant COVID-19 3CLpro protein, N-His tag

Product Description

Cat

IMP-847

Official Symbol

3CLpro

Product Overview

Recombinant COVID-19 3CLpro(YP_009725301.1)(Ser1-Gln306) protein, fused with a 6*His tag at the N-terminus, was expressed from E.coli.

Expression System

E.coli

Species

COVID-19

Tag

N-His tag

Form

Liquid in sterile PBS, 1mM EDTA, 10% Glycerol, pH 7.4.

Molecular Mass

The protein has a calculated MW of 36.1kDa, and migrates as 33kDa under reducing conditions.

Protein length

Ser1-Gln306

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Concentration

Please refer to the COA for detailed information.

SDS-PAGE

Bioactivity-Cell based assay 1

Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans (Synthetic). The specific activity is >13000pmol/min/mg.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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