Recombinant COVID-19 Spike RBD protein, C-mFc-His Tag

Product Description

Cat

IMP-2644

Official Symbol

Spike

Product Overview

Recombinant COVID-19 Spike protein(319-541aa), fused with C-mFc-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

COVID-19

Tag

C-mFc-His Tag

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.

Molecular Mass

The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide.

Protein length

319-541aa

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Biological Activity

ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.

Storage

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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