Recombinant COVID-19 Spike RBD protein, C-mFc-His Tag

Product Description

Cat

IMP-2644

Official Symbol

Spike

Product Overview

Recombinant COVID-19 Spike protein(319-541aa), fused with C-mFc-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

COVID-19

Tag

C-mFc-His Tag

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.

Molecular Mass

The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide.

Protein length

319-541aa

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Biological Activity

ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.

Storage

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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