Recombinant COVID-19(Alpha) Spike RBD protein, C-hFc Tag

Product Description

Cat

IMP-2846

Official Symbol

Spike

Product Overview

Recombinant COVID-19(Alpha) Spike RBD protein(319-541aa), fused with C-terminal hFc Tag, was expressed in HEK293.

Expression System

HEK293

Species

COVID-19

Tag

C-hFc Tag

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Molecular Mass

The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of RBD(N501Y)-hFc is approximately 55-70 kDa due to glycosylation.

Protein length

319-541aa

Purity

The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.

Biological Activity

ELISA plate pre-coated by 2 μg/mL (100 μL/well) SARS-CoV-2 (Alpha) S protein RBD, hFc Tag can bind Human ACE2 Protein, mFc Tag in a linear range of 4.115–1000 ng/mL.

Storage

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry