Recombinant Cynomolgus CD3D&CD3E Protein, No Tag
Product Description
Cat
IMP-4229
Official Symbol
CD3D&CD3E
Product Overview
Recombinant cynomolugs CD3D (Q95LI8-1)(Met1-Ala105) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant cynomolgus CD3E (XP_015290838.1) (Met1-Asp117) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Cynomolgus
Tag
No Tag
Predicted N Terminal
Phe 22&Gln 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of cynomolgus CD3D&CD3E comprises 679 (334+345) amino acids and has a calculated molecular mass of 76.63 (37.75+ 38.88) KDa. The apparent molecular mass of rhesus CD3D&CD3E heterodimer is approximately (50.99&40.83) KDa respectively in SDS-PAGE.
Protein length
Met1-Ala105 and Met1-Asp117
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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