Recombinant Cynomolgus FCGR2A Protein, C-His&C-AVI-tagged

Product Description

Cat

IMP-1985

Official Symbol

FCGR2A

Product Overview

Recombinant Cynomolgus (Macaca fascicularis) CD32a (AAL92096.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His&C-AVI

Predicted N Terminal

Ala 30

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant cynomolgus CD32a consists of 206 amino acids and has a calculated molecular mass of 22.9 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein length

Met1-Gly220

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-BLI 1

Labeled biotin to CD32A Protein, Cynomolgus, Recombinant (His&AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Cynomolgus, Recombinant (His&AVI Tag), Biotinylated bound to IgG1 Antibody was 6.3 nM.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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