Recombinant Cynomolgus FCGR2B Protein, C-His&C-AVI-tagged, Biotinylated

Product Description

Cat

IMP-1998

Official Symbol

FCGR2B

Product Overview

Recombinant Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His&C-AVI

Predicted N Terminal

Ala 45

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein length

Met1-Gly220

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Cynomolgus CD32B/Fcgr2b Protein (His&AVI Tag), Biotinylated at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala), the EC50 is 4-36 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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