Recombinant Cynomolgus FCGRT&B2M Protein, C-His-tagged, Biotinylated
Product Description
Cat
IMP-4486
Official Symbol
FCGRT&B2M
Product Overview
Recombinant extracellular domain (Met1-Ser297) of cyno FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant cyno B2M (Q8SPW0) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified protein was biotinylated in vitro.
Description
This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Cynomolgus
Tag
C-His
Predicted N Terminal
Ala 24&Ile 21
Form
Lyophilized from sterile PBS, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of cyno FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa.
Protein length
Met1-Ser297 and Met1-Met119
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of FCGRT&B2M Heterodimer Protein, Cynomolgus, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 4.6 nM.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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