Recombinant Cynomolgus IL4RA(G7Q0S7)(Met26-Arg232) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Cynomolgus
Tag
C-hFc tag
Form
Lyophilized from sterile 20mM Tris-HCl, 100mM Glycine, pH 7.5.
Protein length
Met26-Arg232
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Bioactivity-BLI 2
Loaded Human IL-4-His on HIS1K Biosensor, can bind Cynomolgus IL-4RA-Fc with an affinity constant of 0.03 nM as determined in BLI assay.
