Recombinant Cynomolgus LAG3 protein, N-His Tag
Product Description
Cat
IMP-511
Official Symbol
LAG3
Product Overview
Recombinant Cynomolgus LAG3(XP_005570011.1)(Pro23-Leu450) is expressed in HEK293, fused with a 8His tag at the N-terminus.
Description
Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.
Expression System
HEK293
Species
Cynomolgus
Tag
N-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.
Molecular Mass
55-65&140kDa, reducing conditions.
Protein length
Pro23-Leu450
Endotoxin
Less than 0.1 ng/μg (1 EU/μg).
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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