Recombinant Cynomolgus LAG3 protein, N-His Tag
Product Description
Cat
IMP-511
Official Symbol
LAG3
Product Overview
Recombinant Cynomolgus LAG3(XP_005570011.1)(Pro23-Leu450) is expressed in HEK293, fused with a 8His tag at the N-terminus.
Description
Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.
Expression System
HEK293
Species
Cynomolgus
Tag
N-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.
Molecular Mass
55-65&140kDa, reducing conditions.
Protein length
Pro23-Leu450
Endotoxin
Less than 0.1 ng/μg (1 EU/μg).
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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