Recombinant Cynomolgus monkey ACE2 protein, C-hFc Tag

Product Description

Cat

IMP-6021

Official Symbol

ACE2

Product Overview

Recombinant Cynomolgus monkey ACE2 protein(A0A2K5X283)(18-741aa), fused with C-terminal hFc tag, was expressed in HEK293.

Expression System

HEK293

Species

Cynomolgus monkey

Tag

C-hFc Tag

Form

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Molecular Mass

112.7 kDa

Protein length

18-741aa

AA Sequence

QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYKDYGDYWRGNYEVNGVDGYDYNRDQLIEDVERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLLNMLKLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRTYFLEIKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRISRSRINDAFRLNDNSLEFLGIQTTLAPPYQSPVTT

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 93% as determined by SDS-PAGE.

Biological Activity

①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 ug/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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