Recombinant Cynomolgus PDCD1 protein, C-Fc Tag

Product Description

Cat

IMP-274

Official Symbol

PDCD1

Product Overview

Recombinant Cynomolgus PDCD1 protein(AA Leu 25-Gln 167)(Accession # B0LAJ3) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus

Tag

C-Fc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.6 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA 1

Immobilized Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Cynomolgus PD-1, Fc Tag with a linear range of 3-33 ng/mL.

Bioactivity-ELISA 2

Immobilized Cynomolgus PD-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.

Bioactivity-BLI 1

Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag with an affinity constant of 1.7 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.4 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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