Cynomolgus PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Cynomolgus PD-1, Fc Tag with a linear range of 3-33 ng/mL.
Immobilized Cynomolgus PD-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.
Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag with an affinity constant of 1.7 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.4 uM as determined in BLI assay (ForteBio Octet Red96e).