Recombinant Cynomolgus PDCD1 protein, C-His Tag

Product Description

Cat

IMP-275

Official Symbol

PDCD1

Product Overview

Recombinant Cynomolgus PDCD1 protein(AA Leu 25-Gln 167)(Accession # B0LAJ3) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Sintilimab with a linear range of 0.4-6 ng/mL.

Bioactivity-ELISA 2

Immobilized Cynomolgus PD-1, His Tag at 1 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag with a linear range of 0.156-2.5 ug/mL.

Bioactivity-BLI 1

Loaded Opdivo (Nivolumab) on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag with an affinity constant of 17.5 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Cynomolgus / Rhesus macaque PD-L1, Fc Tag on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag with an affinity constant of 3.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Opdivo(Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Cynomolgus PD-1, His Tag with an affinity constant of 18.1 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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