Cynomolgus PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cynomolgus PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Sintilimab with a linear range of 0.4-6 ng/mL.
Immobilized Cynomolgus PD-1, His Tag at 1 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag with a linear range of 0.156-2.5 ug/mL.
Loaded Opdivo (Nivolumab) on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag with an affinity constant of 17.5 nM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Cynomolgus / Rhesus macaque PD-L1, Fc Tag on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag with an affinity constant of 3.1 uM as determined in BLI assay (ForteBio Octet Red96e).
Opdivo(Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Cynomolgus PD-1, His Tag with an affinity constant of 18.1 nM as determined in a SPR assay (Biacore T200).
