Recombinant Cynomolgus PDCD1 protein, C-His Tag
Product Description
Cat
IMP-519
Official Symbol
PDCD1
Product Overview
Recombinant Cynomolgus PDCD1(NP_001271065.1)(Pro21-Gln167) is expressed in HEK293, fused with a 6His tag at the C-terminus.
Description
Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Expression System
HEK293
Species
Cynomolgus
Tag
C-His Tag
Form
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Molecular Mass
30-50 kDa, reducing conditions.
Protein length
Pro21-Gln167
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Loaded Anti-Human PD-1 mAb-Fc on Protein A Biosensor, can bind Cynomolgus PD-1-His with an affinity constant of 2.47 nM as determined in BLI assay.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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