Recombinant Cynomolgus PVRL4 protein, C-His tag

Product Description

Cat

IMP-789

Official Symbol

PVRL4

Product Overview

Recombinant Cynomolgus PVRL4(L0N6D9)(Gly31-Val351) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His tag

Form

Lyophilized from sterile 20mM PB, 150mM NaCl, pH 7.4.

Molecular Mass

The protein has a calculated MW of 34.9kDa, and migrates as 38-50kDa under reducing conditions.

Protein length

Gly31-Val351

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-ELISA 1

Immobilized Cynomolgus Nectin-4-His at 5μg/ml (100 μl/well) can bind Human Nectin-1-His: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His is 1.52 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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