Recombinant Cynomolgus / Rhesus macaque CD80 protein, C-Fc Tag

Product Description

Cat

IMP-282

Official Symbol

CD80

Product Overview

Recombinant Cynomolgus / Rhesus macaque CD80 protein(AA Val 35-Asn 242)(Accession # G7NXN7) is expressed from human 293 cells (HEK293). In the region Val 35-Asn 242, the AA sequence of Cynomolgus and Rhesus macaque B7-1 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus / Rhesus macaque

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.5 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus / Rhesus macaque B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag with a linear range of 1-8 ng/mL.

Bioactivity-BLI 1

Loaded Cynomolgus / Rhesus macaque B7-1, Fc Tag on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 4.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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