Recombinant Cynomolgus / Rhesus macaque CD80 protein, C-His Tag

Product Description

Cat

IMP-283

Official Symbol

CD80

Product Overview

Recombinant Cynomolgus / Rhesus macaque CD80 protein(AA Val 35-Asn 242)(Accession # G7NXN7) is expressed from human 293 cells (HEK293). In the region Val 35-Asn 242, the AA sequence of Cynomolgus and Rhesus macaque B7-1 are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus / Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Val 35-Asn 242

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus / Rhesus macaque B7-1, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Cynomolgus / Rhesus macaque B7-1, His Tag (HPLC-verified) was more than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag at 5 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, His Tag (HPLC-verified) with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Immobilized Human CTLA-4, Fc Tag at 5 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, His Tag (HPLC-verified) with a linear range of 10-156 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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