Recombinant Cynomolgus / Rhesus macaque CD86 protein, C-Fc Tag
Product Description
Cat
IMP-280
Official Symbol
CD86
Product Overview
Recombinant Cynomolgus / Rhesus macaque CD86 protein(AA Ala 19-His 240)(Accession # G7NXR4) is expressed from human 293 cells (HEK293). In the region Ala 19-His 240, the AA sequence of Cynomolgus and Rhesus macaque B7-2 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Cynomolgus / Rhesus macaque
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52 kDa. The protein migrates as 80-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Ala 19-His 240
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Cynomolgus / Rhesus macaque B7-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-2, Fc Tag with a linear range of 0.039-1.25 ug/mL.

Bioactivity-BLI 1

Loaded Cynomolgus / Rhesus macaque B7-2, Fc Tag on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag with an affinity constant of 11 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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