Recombinant Cynomolgus / Rhesus macaque CD86 protein, C-His Tag

Product Description

Cat

IMP-281

Official Symbol

CD86

Product Overview

Recombinant Cynomolgus / Rhesus macaque CD86 protein(AA Ala 19-His 240)(Accession # G7NXR4-1) is expressed from human 293 cells (HEK293). In the region Ala 19-His 240, the AA sequence of Cynomolgus and Rhesus macaque B7-2 are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus / Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.3 kDa. The protein migrates as 50-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ala 19-His 240

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) was more than 95% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque CTLA-4, Fc Tag at 5 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) with a linear range of 0.312-5 ug/mL.

Bioactivity-ELISA 2

Immobilized Cynomolgus / Rhesus macaque B7-2, His Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag with a linear range of 0.02-0.625 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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