Recombinant Cynomolgus / Rhesus macaque TNFRSF9 protein, C-His Tag

Product Description

Cat

IMP-284

Official Symbol

TNFRSF9

Product Overview

Recombinant Cynomolgus / Rhesus macaque TNFRSF9 protein(AA Leu 24-Gln 186)(Accession # XP_005544945.1) is expressed from human 293 cells (HEK293). In the region Leu 24-Gln 186, the AA sequence of Cynomolgus and Rhesus macaque 4-1BB are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus / Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE).

Protein length

Leu 24-Gln 186

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus / Rhesus macaque 4-1BB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque 4-1BB, His Tag at 1 ug/mL (100 uL/well) can bind Human 4‑1BB Ligand, Fc Tag with a linear range of 0.05-0.5 ng/mL.

Bioactivity-BLI 1

Loaded Human 4-1BB Ligand (71-254), Fc Tag (active trimer) (MALS verified) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque 4-1BB, His Tag with an affinity constant of 10.3 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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