Recombinant Cynomolgus SIGLEC15 protein, C-Fc Tag

Product Description

Cat

IMP-559

Official Symbol

SIGLEC15

Product Overview

Recombinant Cynomolgus SIGLEC15(XP_005586866.1)(Phe20-Thr263) is expressed in HEK293, fused with a Fc tag at the C-terminus.

Description

Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Expression System

HEK293

Species

Cynomolgus

Tag

C-Fc Tag

Form

Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.

Molecular Mass

55-70kDa, reducing conditions.

Protein length

Phe20-Thr263

Endotoxin

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Cynomolgus Siglec-15-Fc at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 20.9 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Cynomolgus Siglec-15-Fc with an affinity constant of 0.16 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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