Recombinant Cynomolgus SIGLEC15 protein, C-Fc Tag

Product Description

Cat

IMP-559

Official Symbol

SIGLEC15

Product Overview

Recombinant Cynomolgus SIGLEC15(XP_005586866.1)(Phe20-Thr263) is expressed in HEK293, fused with a Fc tag at the C-terminus.

Description

Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Expression System

HEK293

Species

Cynomolgus

Tag

C-Fc Tag

Form

Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.

Molecular Mass

55-70kDa, reducing conditions.

Protein length

Phe20-Thr263

Endotoxin

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Cynomolgus Siglec-15-Fc at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 20.9 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Cynomolgus Siglec-15-Fc with an affinity constant of 0.16 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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