Recombinant Cynomolgus SIRPA protein, C-His Tag

Product Description

Cat

IMP-142

Official Symbol

SIRPA

Product Overview

Recombinant Cynomolgus SIRPA protein(AA Glu 31-Asn 370)(Accession # NP_001271679.1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 31-Asn 370

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.313-2.5 ug/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Human CD47, Fc,Avitag at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2ug/well) plate, can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-0.625 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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