Recombinant Cynomolgus SIRPA protein, C-His Tag

Product Description

Cat

IMP-142

Official Symbol

SIRPA

Product Overview

Recombinant Cynomolgus SIRPA protein(AA Glu 31-Asn 370)(Accession # NP_001271679.1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 31-Asn 370

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.313-2.5 ug/mL.

Bioactivity-ELISA 2

Immobilized Biotinylated Human CD47, Fc,Avitag at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2ug/well) plate, can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-0.625 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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