Recombinant Cynomolgus TIGIT Protein, C-hFc-tagged

Product Description

Cat

IMP-1377

Official Symbol

TIGIT

Product Overview

Recombinant cynomolgus TIGIT (XP_015300911.1) (Met1-Pro209) was expressed with the Fc region of human IgG1 at the C-terminus.

Description

TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

Expression System

HEK293

Species

Cynomolgus

Tag

C-hFc

Predicted N Terminal

Lys 27

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant cynomolgus TIGIT consists of 421 amino acids and predicts a molecular mass of 47.2 kDa.

Protein length

Met1-Pro209

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized human CD155/PVR Protein, Human, Recombinant (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind TIGIT Protein, Cynomolgus, Recombinant (ECD, hFc Tag), the EC50 of TIGIT Protein, Cynomolgus, Recombinant (ECD, hFc Tag) is 5-30 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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