Recombinant Cynomolgus/Rhesus macaque CD27 protein, C-mFc Tag
Product Description
Cat
IMP-167
Official Symbol
CD27
Product Overview
Recombinant Cynomolgus/Rhesus macaque CD27 protein(AA Ala 20-Arg 191)(Accession # XP_005569963.1) is expressed from human 293 cells (HEK293). In the region Ala 20-Arg 191, the AA sequence of Cynomolgus and Rhesus macaque CD27 are homologus. This protein carries a mouse IgG2a Fc tag at the C-terminus.
Expression System
HEK293
Species
Cynomolgus/Rhesus macaque
Tag
C-mFc Tag
Form
Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Ala 20-Arg 191
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 2-31 ng/mL (QC tested).

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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