Recombinant Cynomolgus/Rhesus macaque CD274 protein, C-His Tag

Product Description

Cat

IMP-273

Official Symbol

CD274

Product Overview

Recombinant Cynomolgus/Rhesus macaque CD274 protein(AA Phe 19-Arg 238)(Accession # G7PSE7-1) is expressed from human 293 cells (HEK293). In the region Phe 19-Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus/Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.1 kDa. The protein migrates as 37-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus/Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus/Rhesus macaque PD-L1, His Tag at 1 ug/mL (100 uL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL (QC tested).

Bioactivity-BLI 1

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-BLI 2

Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 1.7 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-SPR 1

Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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