Recombinant Cynomolgus/Rhesus macaque CD274 protein, C-His Tag

Product Description

Cat

IMP-273

Official Symbol

CD274

Product Overview

Recombinant Cynomolgus/Rhesus macaque CD274 protein(AA Phe 19-Arg 238)(Accession # G7PSE7-1) is expressed from human 293 cells (HEK293). In the region Phe 19-Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus/Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.1 kDa. The protein migrates as 37-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Arg 238

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus/Rhesus macaque PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Cynomolgus/Rhesus macaque PD-L1, His Tag at 1 ug/mL (100 uL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.4-3 ng/mL (QC tested).

Bioactivity-BLI 1

Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-BLI 2

Loaded Cynomolgus PD-1, Fc Tag on Protein A Biosensor, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 1.7 uM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-SPR 1

Anti-Human PD-L1 MAb captured on CM5 chip via anti-human IgG Fc antibody surface, can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 23.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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