Recombinant Cynomolgus/Rhesus macaque CD47 protein, C-Fc Tag

Product Description

Cat

IMP-278

Official Symbol

CD47

Product Overview

Recombinant Cynomolgus/Rhesus macaque CD47 protein(AA Gln 19-Glu 141)(Accession # F7A802-1) is expressed from human 293 cells (HEK293). In the region Gln 19-Glu 141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus/Rhesus macaque

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. As a result of glycosylation, the protein migrates as 50-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-P

Protein length

Gln 19-Glu 141

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus/Rhesus macaque CD47, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag at 10 ug/mL (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, Fc Tag with a linear range of 0.039-0.625 ug/mL (QC tested).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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