Recombinant Cynomolgus/Rhesus macaque CD47 protein, C-Fc Tag
Product Description
Cat
IMP-278
Official Symbol
CD47
Product Overview
Recombinant Cynomolgus/Rhesus macaque CD47 protein(AA Gln 19-Glu 141)(Accession # F7A802-1) is expressed from human 293 cells (HEK293). In the region Gln 19-Glu 141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Cynomolgus/Rhesus macaque
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. As a result of glycosylation, the protein migrates as 50-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-P
Protein length
Gln 19-Glu 141
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Cynomolgus/Rhesus macaque CD47, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag at 10 ug/mL (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, Fc Tag with a linear range of 0.039-0.625 ug/mL (QC tested).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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