Recombinant Cynomolgus/Rhesus macaque CD47 protein, C-His Tag

Product Description

Cat

IMP-173

Official Symbol

CD47

Product Overview

Recombinant Cynomolgus/Rhesus macaque CD47 protein(AA Gln 19-Glu 141)(Accession # F7A802-1) is expressed from human 293 cells (HEK293). In the region Gln 19-Glu 141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus. This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus/Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gln 19-Glu 141

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus/Rhesus macaque CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 4-63 ng/mL (QC tested).

Bioactivity-ELISA 2

Immobilized Anti-CD47, Human IgG4 at 2 ug/mL (100 uL/well) can bind Cynomolgus/Rhesus macaque CD47, His Tag with a linear range of 0.2-5 ng/mL (Routinely tested).

Bioactivity-BLI 1

Loaded Anti-Human CD47 MAb (Human IgG4) on AHC Biosensor, can bind Cynomolgus/Rhesus macaque CD47, His Tag with an affinity constant of 2.93 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-SPR 1

Anti-CD47 Mab (Human IgG4) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Cynomolgus/Rhesus macaque CD47, His Tag with an affinity constant of 1.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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