Recombinant Cynomolgus/Rhesus macaque CTLA4 protein, C-Fc Tag
Product Description
Cat
IMP-276
Official Symbol
CTLA4
Product Overview
Recombinant Cynomolgus/Rhesus macaque CTLA4 protein(AA Ala 37-Ser 160)(Accession # G7PL88-1) is expressed from human 293 cells (HEK293). In the region Ala 37-Ser 160, the AA sequence of Cynomolgus and Rhesus macaque CTLA-4 are homologus. This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Cynomolgus/Rhesus macaque
Tag
C-Fc Tag
Form
Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. As a result of glycosylation, the protein migrates as 45-52 kDa under reducing (R) condition, and 90-105 kDa under non-reducing (NR) condition (SDS-PA
Protein length
Ala 37-Ser 160
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Cynomolgus/Rhesus macaque CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Cynomolgus/Rhesus macaque CTLA-4, Fc Tag at 5 ug/mL (100 uL/well)can bind Cynomolgus/Rhesus macaque B7-2, His Tag with a linear range of 0.312-5 ug/mL (QC tested).

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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