Recombinant Cynomolgus/Rhesus macaque TIGIT protein, C-His Tag

Product Description

Cat

IMP-003

Official Symbol

TIGIT

Product Overview

Recombinant Cynomolgus/Rhesus macaque TIGIT protein(AA Met 89-Ile 208)(Accession # XP_015300911.1) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Cynomolgus/Rhesus macaque

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.2 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Met 89-Ile 208

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Cynomolgus / Rhesus macaque TIGIT, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

HPLC

The purity of Cynomolgus / Rhesus macaque TIGIT, His Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Cynomolgus / Rhesus macaque TIGIT, His Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Human CD155, Fc Tag with a linear range of 0.078-2.5 ug/mL.

Bioactivity-BLI 1

Loaded Human CD155, Fc Tag on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque TIGIT, His Tag (HPLC-verified) with an affinity constant of 0.61 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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