Recombinant Dog IL1A Protein (109-265aa), C-His-Tagged
Product Description
Cat
IMP-9522
Official Symbol
IL1A
Product Overview
Recombinant canine IL-1 alpha/IL-1F1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
IL-1 alpha/IL-1F1, also known as hematopoietin 1 is a cytokine of the interleukin 1 family. IL-1α is a unique member in the cytokine family in the sense that the structure of its initially synthesized precursor does not contain a signal peptide fragment. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1 alpha inhibitors are being developed to interrupt those processes and treat diseases. IL-1 alpha is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation.
Expression System
Baculovirus
Species
Dog
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
19.3 kDa (166 aa)
Protein length
109-265
AA Sequence
<ADP>SVAYNFHNNEKYNYIRIIKSQFILNDNLNQSIVRQTGGNYLMTAALQNLDDAVKFDMGAYTSEDSKLPVTLRISKTRLFVSAQNEDEPVLLKEMPETPKTIRDETNLLFFWERHGSKHYFKSVAQPKLFIATQERKLVHMARGQPSITDFRLLETQP<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.25 mg/mL (determined by Absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Canine IL-1 alpha/IL-1F1 in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range ≤ 2 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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