Recombinant Dog IL1B Protein (114-265aa), Tag Free

Product Description

Cat

IMP-9517

Official Symbol

IL1B

Product Overview

Recombinant canine IL-1 beta was expressed in E. coli and purified by using conventional chromatography techniques.

Description

Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid.

Expression System

E. coli

Species

Dog

Tag

Tag Free

Form

Liquid in Phosphate-Buffered Saline (pH 7.4)

Molecular Mass

17.5 kDa (153 aa) confirmed by MALDI-TOF

Protein length

114-265

AA Sequence

MAAMQSVDCKLQDISHKYLVLSNSYELRALHLNGENVNKQVVFHMSFVHGDESNNKIPVVLGIKQKNLYLSCVMKDGKPTLQLEKVDPKVYPKRKMEKRFVFNKIEIKNTVEFESSQYPNWYISTSQVEGMPVFLGNTRGGQDITDFTMEFSS

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/mL (determined by absorbance at 280nm)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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