Recombinant Dog IL2 (C147S) Protein (21-155aa), N-His-Tagged

Product Description

Cat

IMP-9428

Official Symbol

IL2

Product Overview

Recombinant canine IL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Description

IL2, also known as Interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytres, macrophages and oligodendrocytes.

Expression System

E. coli

Species

Dog

Tag

N-His

Form

Liquid in Phosphate-Buffered Saline (pH 7.4)

Molecular Mass

18.1 kDa (160 aa) confirmed by MALDI-TOF

Protein length

21-155

AA Sequence

<MGSSHHHHHHSSGLVPRGSHMGSHM>APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE, Bioactivity

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5 mg/mL (determined by Bradford assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1

Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range ≤ 0.3ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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