Recombinant Dog IL2 (C147S) Protein (21-155aa), N-His-Tagged
Product Description
Cat
IMP-9428
Official Symbol
IL2
Product Overview
Recombinant canine IL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Description
IL2, also known as Interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytres, macrophages and oligodendrocytes.
Expression System
E. coli
Species
Dog
Tag
N-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4)
Molecular Mass
18.1 kDa (160 aa) confirmed by MALDI-TOF
Protein length
21-155
AA Sequence
<MGSSHHHHHHSSGLVPRGSHMGSHM>APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/mL (determined by Bradford assay)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range ≤ 0.3ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
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  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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